Fig. 7.

Polydatin inhibits NF-κB activation in HG-induced H9c2 cells. H9c2 cells were cultured in DMEM containing 30 mmol/L glucose (HG) or 5.5 mmol/L glucose (normal glucose) and treated with polydatin (40 μmol/L), DPI (a NADPH oxidase inhibitor, 10 μmol/L) or polydatin (40 μmol/L) + DPI (10 μmol/L) for 24 h. The expression of TNF-α, IL-1β, IL-6 and VCAM-1 was determined by ELISA. NF-κB expression was assessed by Western blot. a IL-1β levels in H9c2 cells. b IL-6 levels in H9c2 cells. c TNF-α levels in H9c2 cells. d VCAM-1 levels in H9c2 cells. e The expression of p65 in nucleus of H9c2 cells. f The expression of phosphorylation of IκB-α in cytoplasm of H9c2 cells. g Representative immunoblots of p65 and phosphorylation of IκB-α protein levels in H9c2 cells. Results were expressed as means ± SEM (n = 6). *P < 0.05, **P < 0.01 versus control values; #P < 0.05, ##P < 0.01versus HG