Table I.
MRM Transitions, Cone Voltages, Collision Energies and Retention Times for Compounds and Internal Standards (IS)
| Compound | MRM transitions (m/z) | Cone voltage (V) | Collision energy (eV) | Retention time (min) | IS used |
|---|---|---|---|---|---|
| Clonazolam | 354.0 > 308.0/326.0 | 40 | 20 | 2.11 | Etizolam-d3/ nitrazepam-d5 |
| Diclazepam | 321.0 > 154.0/227.0 | 40 | 31/33 | 5.38 | Diclazepam-d4/ nitrazepam-d5 |
| Etizolam | 343.0 > 314.0/206.0 | 40 | 26/24 | 3.99 | Etizolam-d3/ nitrazepam-d5 |
| Flualprazolam | 327.2 > 299.1/292.1 | 45 | 25 | 3.80a (2.81)b | Diazepam-d5 |
| Flubromazepam | 335.0 > 186.0/179.0 | 45 | 45/40 | 3.67 | Flunitrazepam-d7 |
| Flubromazolam | 371.0 > 292.0/343.0 | 60 | 31/37 | 3.06 | Etizolam-d3/ nitrazepam-d5 |
| Phenazepam | 351.1 > 179.1/206.1 | 50 | 40/37 | 4.24 | Flunitrazepam-d7 |
| Diazepam-d5 | 290.1 > 198.1 | 40 | 30 | 4.24a | |
| Diclazepam-d4 | 325.0 > 154.0 | 40 | 30 | 5.33 | |
| Etizolam-d3 | 348.0 > 319.0 | 40 | 24 | 3.97 | |
| Flunitrazepam-d7 | 321.1 > 275.1 | 35 | 25 | 3.05 | |
| Nitrazepam-d5 | 287.0 > 185.0 | 50 | 30 | 2.25 |
a
Flualprazolam and diazepam-d5 were analysed with a different method than the other compounds given in the table.
b
Flualprazolam has been added to the same method as the other compounds at a later date, and the corresponding retention time is given in parenthesis for comparison.