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. 2020 Dec 8;12:12603–12612. doi: 10.2147/CMAR.S253452

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© 2020 Li et al.

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NLIPMT upregulated survivin expression in tumor cells through miR-320. (A–D) NLIPMT and survivin expression vectors as well as miR-320 mimic were transfected into KYSE510 cells. (A) The overexpression of NLIPMT, miR-320 and survivin was confirmed by qRT-PCR. (B) The interaction between NLIPMT and miR-320 was analyzed by qRT-PCR. (C) The mRNA level of survivin was detected by qRT-PCR. (D) The protein level of survivin was detected by Western blotting. (E) miR-329 inhibitor was transfected into KYSE510 cells. miR-320 level was detected by qRT-PCR. (F) NLIPMT expression vector and miR-329 inhibitor were co-transfected into KYSE510 and ECA109 cells, and survivin, protein was measured by Western blotting. *p < 0.05.