Table 1.

Measured Responses of Box–Behnken Design (Mean ±SD, n=3)

Trial	Factors			Responses
	A: SPC Molar Conc. (M)	B: SDC Amount (mg)	C: Limonene Percentage (%)	Y1: %EE	Y2: PS (nm)	Y3: PDI	Y4: ZP (mV)	Y5: % in vitro Release (2 h)	Y6: % in vitro Release (10 h)
T1	0.02	20	0.25	67.86±2.42	172.1±7.21	0.43±0.01	−37.5±0.42	33.70±3.27	65.23±2.43
T2	0.02	10	0.5	69.32±1.69	155.5±6.65	0.39±0.03	−34.43±0.42	34.44±6.59	66.99±4.72
T3	0.02	30	0.5	65.46±4.24	166.2±4.95	0.32±0.06	−40.4±2.40	36.11±5.41	68.33±1.34
T4	0.02	20	0.75	57.11±0.81	160.3±9.19	0.20±0.01	−39.3±1.56	38.84±3.27	68.61±6.17
T5	0.04	30	0.25	67.53±5.24	207.6±3.25	0.31±0.08	−39.2±3.11	24.90±4.65	41.48±2.39
T6	0.04	30	0.75	67.38±3.14	205.1±5.66	0.36±0.03	−40.1±0.85	37.88±1.24	58.47±3.20
T7	0.04	10	0.25	75.34±1.45	188.3±4.81	0.37±0.04	−32.6±1.27	32.51±5.17	42.16±4.53
T8	0.04	20	0.5	70.76±0.73	198.1±1.98	0.40±0.03	−35.7±2.40	35.59±3.23	58.45±1.67
T9	0.04	20	0.5	71.58±5.70	197.7±1.41	0.32±0.04	−37.4±0.99	32.20±1.19	48.16±4.56
T10	0.04	10	0.75	70.16±4.22	185.6±2.40	0.38±0.01	−34.3±0.85	35.58±2.65	55.50±4.62
T11	0.04	20	0.5	72.30±1.88	189.6±2.69	0.31±0.01	−34.8±1.70	31.32±3.09	56.37±1.75
T12	0.06	10	0.5	85.77±1.26	214.1±5.66	0.33±0.06	−21.2±3.25	27.38±1.63	49.05±1.69
T13	0.06	20	0.75	77.43±4.08	221.6±1.70	0.25±0.08	−27.3±0.42	29.83±2.18	58.62±1.53
T14	0.06	30	0.5	75.32±3.14	230.2±2.69	0.28±0.04	−30.1±1.27	28.60±0.83	50.64±3.44
T15	0.06	20	0.25	78.44±2.34	220.9±3.39	0.31±0.08	−25.5±0.71	25.87±4.68	42.81±2.63

Abbreviations: %EE, percent entrapment efficiency; PDI, polydispersity index; PS, particle size; SDC, sodium deoxycholate; SPC, soybean phosphatidyl choline; ZP, zeta potential.