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. 2020 Dec 12;11(12):1055. doi: 10.1038/s41419-020-03257-4

Fig. 7. NFKB1 was enriched on the core promoter region of AC007271.3 and the canonical NF-κB pathway upregulated the expression of AC007271.3.

Fig. 7

a After transfecting fragment-by-fragment deletion pGL3 vectors of AC007271.3 promoters into 293 T cells for 48 hours, the luciferase activity was measured and analyzed statistically. b The binding sequence logo of NFKB1 on JASPAR database. c Enrichment of NFKB1 was detected at the Site 2 (−854/−842) of AC007271.3 core promoter in SCC9 by ChIP-qPCR analysis. d After treating with TNF-α (10 ng/ml) for the indicated times in SCC9, the mRNA level of AC007271.3, miR-125b-2-3p, and Slug was analyzed by qRT-PCR. GAPDH was used as a control. e The western blot analysis indicated that the protein level of Slug also increased by TNF-α treatment (10 ng/ml, 72 hours). α-tubulin was employed as a control. f The canonical NF-κB pathway-related proteins in the cytoplasmic and nuclear of SCC9 which treated with or without TNF-α (10 ng/ml, 72 h) were assessed by western blot. α-tubulin and Histone H3 were employed as the positive controls for cytoplasmic and nuclear proteins, respectively. *p < 0.05, **p < 0.01, ***p < 0.001.