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. 2020 Dec 11;11:6348. doi: 10.1038/s41467-020-20165-5

Fig. 4. LncRNA LUCAT1 is enriched in nuclear compartment upon activation, and is associated with chromatin.

Fig. 4

a RT-qPCR analysis of GAPDH, LUCAT1, and MALAT1 gene expression in nuclear and cytoplasmic fractions in LPS-stimulated THP-1 cells (n = 3, biologically independent experiments). b smFISH analysis for LUCAT1 in primary human hDCs in stimulated conditions. LUCAT1 probes are represented in red, chromatin staining by DAPI in blue, and GAPDH mRNA in green (n = 3, biologically independent experiments) c Quantification of LUCAT1 puncta in smFISH imaging in b (n = 3, biologically independent experiments unpaired two-tailed t-test, p = 0.0099). d RT-qPCR analysis of LUCAT1 and MALAT1 gene expression in RNA immunoprecipitation (RIP) samples using antibody against Histone H3 (n = 4 for LUCAT1 RIP and n = 2 for MALAT1 RIP, biologically independent experiments; unpaired two-tailed t-test, LUCAT1 p = 0.0002; MALAT1 p = 0.0056). Data is represented as mean ± SEM *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001.