Table 1.
Statistics of cryoEM data collection and refinement.
| Name | hnRNPA1 LC fibril |
|---|---|
| PDB ID | 7BX7 |
| EMDB ID | EMD-30235 |
| Data collection | |
| Magnification | 130,000 |
| Pixel size (Å) | 1.04 |
| Defocus Range (μm) | −1.3 to −2.1 |
| Voltage (kV) | 300 |
| Camera | K2 summit |
| Microscope | Titan Krios |
| Exposure time (s/frame) | 0.25 |
| Number of frames | 32 |
| Total dose (e−/Å2) | 59 |
| Reconstruction | |
| Micrographs | 3227 |
| Manually picked fibrils | 62,656 |
| Box size (pixel) | 288 |
| Inter-box distance (Å) | 30.0 |
| Segments extracted | 801,707 |
| Segments after Class2D | 259,615 |
| Segments after Class3D | 69,037 |
| Resolution (Å) | 2.80 |
| Map sharpening B-factor (Å2) | −109.473 |
| Helical rise (Å) | 2.37 |
| Helical twist (°) | 179.05 |
| Atomic model | |
| Non-hydrogen atoms | 1896 |
| Protein residues | 270 |
| Ligands | 0 |
| r.m.s.d. Bond lengths | 0.009 |
| r.m.s.d. Bond angles | 0.833 |
| All-atom clash score | 15.34 |
| Rotamer outliers | 0.00% |
| Ramachandran outliers | 0.00% |
| Ramachandran allowed | 9.30% |
| Ramachandran favored | 90.70% |