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. 2020 Dec 11;11:6341. doi: 10.1038/s41467-020-20191-3

Fig. 5. ALS-associated FUS traps U1 snRNA in the cytoplasm of motor neurons.

Fig. 5

a Schematic representation of the genome-edited hiPSC-derived motor neurons used in this study. b Immunostaining in hiPSC-derived motor neurons with and without sodium arsenite treatment. FUS (green) and Snurportin-1 (red) were visualised using respective antibodies. Nuclei were counterstained with DAPI. Scale bar = 15 µm, n = 2. c Combined FISH and immunostaining in hiPSC-derived motor neurons following sodium arsenite treatment. FUS was visualised using anti-FUS antibodies (red), U1 snRNA with 6-FAM azide-labelled antisense probe (green). Nuclei were counterstained with DAPI. Scale bar = 15 µm, n = 1. d RNA FISH in 18-month spinal cord sections of WT and ‘FUSDelta14’ mice. U1 snRNA was visualised using 6-FAM azide-labelled antisense probe. Scale bar = 75 µm, n = 5. e High-resolution images of motor neurons from the same sections as the images in panel d. Scale bar = 15 µm, n = 5.