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. 2020 Dec 12;11(12):1054. doi: 10.1038/s41419-020-03230-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — A Immunohistochemical staining for fibrin in the tissue of Plg-R_KT^−/− and Plg-R_KT^+/+ mice collected at different time points after induction of burn wounds (n = 4). Scale bar = 200 µm. Images were captured with a Zeiss Axio Imager Z1 (Zeiss, Oberkochen, Germany) or Nikon A1R Eclipse Ti-E inverted microscope (Nikon Instruments, Amsterdam, Netherlands). B Quantification of fibrinogen area in panel (A) (%) based on immunostaining using Image J software. Data analysis by robust ANOVA showed a significant effect for time (p < 0.0001, f = 68.694), a significant effect for genotype (p = 0.0002, f = 20.257) and a significant genotype × time interaction (p < 0.0001, f = 35.604). Post hoc testing by Mann–Whitney test: Day 4, Plg-R_KT^+/+ (Mdn = 13), Plg-R_KT^−/− (Mdn = 3.8), (U = 0, p = 0.029), Hodges–Lehmann median difference = −9.3; Day 11, Plg-R_KT^+/+ (Mdn = 2.3), Plg-R_KT^−/− (Mdn = 5.5), (U = 2, p = 0.114); Day 20, Plg-R_KT^+/+ (Mdn = 0.22), Plg-R_KT^−/− (Mdn = 0.34), (U = 18, p = 0.731); Plg-R_KT^+/+ Day 4 (Mdn = 13.23), Day 11 (Mdn = 2.29), (U = 0, p = 0.029), Hodges–Lehmann median difference = -10.94; Plg-R_KT^−/− Day 4 (Mdn = 3.842), Day 11 (Mdn = 5.500), U = 7, p = 0.886). Plg-R_KT^+/+ Day 4 (Mdn = 13.23), Day 20 (Mdn = 0.221), (U = 0, p = 0.0061), Hodges Lehmann median difference = −12.94); Plg-R_KT^−/− Day 4 (Mdn = 3.842), Day 20 (Mdn = 0.344), (U = 0, p = .0095), Hodges–Lehmann median difference = −3.482. Medians and p values from post hoc testing by Mann–Whitney test is displayed. C Wound tissue obtained at day 11 after wounding or control abdominal tissue was lysed and 30 μg of total protein was electrophoresed under nonreducing conditions and western blotted with anti-fibrin(ogen) and anti-βactin antibodies. D Gels were scanned and quantified densitometrically using LICOR software. By Mann–Whitney test: Plg-R_KT^+/+ (Mdn = 0.42), Plg-R_KT^−/− (Mdn = 0.77), (U = 0, p = 0.100), n = 3. Medians and p values determined by the Mann–Whitney test are displayed. E Quantification of the remaining wound area at different time points after burn-wounding of male Plg-R_KT^−/−/Fgn^+/− (n = 4) mice and Plg-R_KT^+/+/Fgn^+/− mice (n = 5). The mixed-effects model (REML) showed a significant effect of time (p = 0.001, f = 12.94), but no effect of genotype (p = 0.868, f = 0.030) and no genotype × time interaction (p = 0.762), f = 0.463).