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. 2020 Dec 11;10:21795. doi: 10.1038/s41598-020-78464-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Confirmation of isolation of exosomes from human serum, using western blot analyses of CD9 and CD63 levels (A), size distribution (B), and morphology (C). Human serum-derived exosomes were isolated using a kit. (A) The lysate of the resulting exosome fraction was resolved by SDS-PAGE and electroblotted onto a PVDF membrane. The blot was probed with anti CD9- and CD63-antibodies, followed by a corresponding HRP-tagged secondary antibody. Data are representative of three experiments. Full-length blots are present in Supplemental Fig. 1. (B) Isolated exosomes were suspended in 100 µL PBS. The size of the exosomes was measured by dynamic light scattering. (C) The morphology of exosomes was examined by TEM. Scale bar = 100 nm.