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. 2020 Dec 11;10:21795. doi: 10.1038/s41598-020-78464-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Quantification analyses of the association of exosomes or 20-nm nanoparticles with CHO-CT and CHO-MARCO cells. T-REx-CHO-MARCO cells were cultured in a 96-well plate with (CHO-MARCO) or without (CHO-CT) tetracycline. After 24 h of culture, the cells were treated with red fluorescent exosomes or 20-nm red fluorescent nanoparticles for 6 h. After incubation, the cells were washed twice with HBSS and fixed with 4% paraformaldehyde, followed by staining with DAPI and phalloidin. The associated fluorescent amounts were analyzed using an IN Cell Analyzer. Data are presented as means ± SEM (n=3). *, Significantly different from CHO-CT cells.