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. 2020 Dec 12;10:428. doi: 10.1038/s41398-020-01091-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Quantification of Oxtr colocalization with respective marker, expressed as a percentage of total Oxtr-positive cells. n = 6CR/4nNOS/3NPY/3PV/3SOM/3Glur2/3 mice per group. b Quantification of Oxtr colocalization with respective marker, expressed as a percentage of marker-positive cells n = 3–6 mice per group. c Quantification of Oxtr colocalization with total interneuron markers or mossy cell marker, expressed as a percentage of total interneuron marker-positive or Glur2/3-positive, respectively. d Representative images of Oxtr and respective markers of interneurons and mossy cell coexpression in the DG. e Oxtr-positive hilar neurons were identified visually and recorded in voltage-clamp configuration mode. Heterogeneous firing patterns were observed. f Summary plot of all reconstructed axonal (blue) and dendritic (red) distribution patterns of Oxtr-HI merged into a map of the hippocampus. Note axonal arborization covering the entire DG. Also notice the presence of individual collaterals exiting the DG and projecting to area CA3 of the hippocampal formation. Bottom inset, quantitative analysis of the dendritic, bottom left, and axonal, bottom right, domains, as shown in contour plot indicating the density of either the dendritic and axonal segments with respect to their position in the hippocampus. Note that the dendritic domains are mainly restricted to the layer of the origin of somata (red dots). Oxtr-HI axons show a distributed density pattern with projections into the dentate granule cell layer, stratum moleculare, and CA3 stratum oriens, stratum lucidum, stratum radiatum, and stratum lacunosum moleculare. Voxel size 50 μm³. g Representative widefield florescent microscopy images showing TdT-positive cell bodies following injection of Cre-dependent transsynaptic anterograde tracer in the hilus of Oxtr-Cre mice. Note the presence of TdT-positive cell bodies in the DG GCL, and modest expression in the hilus and CA3 stratum pyramidale. Quantification of TdT-positive neurons in the suprapyramidal and infrapyramidal DG blades, respectively. A difference was observed, with significantly more TdT-positive cell bodies in the suprapyramidal blade. n = 8 mice; t = 2.836, df = 30, P = 0.0081. h TdT-positive cell bodies in the GCL were rarely colocalized with GAD65 positive neurons. Scale bars: 250 μm. **P < 0.01. ML moleculare layer, GCL granule cell layer, Hil Hilus, SP stratum pyramidale.