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. 2020 Dec 12;11(12):1057. doi: 10.1038/s41419-020-03267-2

Fig. 6. HDAC inhibitor upregulated PSTPIP2 expression in vivo and in vitro.

Fig. 6

a The annotation information of H3K27Ac locus from the UCSC genome browser. b Chromatin immunoprecipitation assay of acetylated histone in the PSTPIP2 promoter region. c Protein expression of H3K27Ac in kidney tissue detected by western blot. d Protein expression of H3K27Ac in CP-treated HK-2 cells detected by western blot. e Representative images of H&E staining of the kidney sections with CP-induced acute kidney injury (AKI) in mice treated with TSA. Scale bars, 50 μM and 20 μM; magnification, 20× and 40×. Similar results were obtained in 3 independent experiments with 10 mice per group or in triplicate culture assays.