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. 2020 Dec 12;11(12):1060. doi: 10.1038/s41419-020-03282-3

Fig. 5. FZD5 maintains stem cell-like properties.

Fig. 5

A CD133, EPCAM, ALDH1A2, and POU5F1 expression in MDA-MB-231 cells was detected by real-time PCR. B Fractions of CD133-positive and EPCAM-positive MDA-MB-231 cells were detected by flow cytometry. C Mammosphere formation of MDA-MB-231 cells was shown. Scale bar: 500 μm, mean ± SD, n = 3. *P < 0.05, **P < 0.01, ***P < 0.001, vs. shCtrl. D CD133, EPCAM, ALDH1A2, and POU5F1 expression in Hs-578t cells was detected by real-time PCR. E Fractions of CD133-positive and EPCAM-positive Hs-578t cells were detected by flow cytometry. F Mammosphere formation of Hs-578t cells was shown. Scale bar: 500 μm, mean ± SD, n = 3. *P < 0.05, **P < 0.01, ***P < 0.001, vs. OE Ctrl.