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. 2020 Dec 12;11(12):1060. doi: 10.1038/s41419-020-03282-3

Fig. 7. FOXM1 overexpression induces FZD5-associated phenotype.

Fig. 7

A Viability of MDA-MB-231 cells was detected by CCK8. B Cell cycle of MDA-MB-231 cells was analyzed by flow cytometry. C γ-H2AX expression in MDA-MB-231 cells was detected by immunofluorescence 48 h after treatment with ADR (300 nM). Scale bar: 100 μm. D Mammosphere formation of MDA-MB-231 cells was shown. Scale bar: 500 μm, mean ± SD, n = 3. **P < 0.01, ***P < 0.001, vs. shCtrl; #P < 0.05, ##P < 0.01, vs. shFZD5-1. E Viability of Hs-578t cells was detected by CCK8. F Cell cycle of Hs-578t cells was analyzed by flow cytometry. G γ-H2AX expression in Hs-578t cells was detected by immunofluorescence 48 h after treatment with ADR (300 nM). Scale bar: 100 μm. H Mammosphere formation of Hs-578t cells was shown. Scale bar: 500 μm, mean ± SD, n = 3. *P < 0.05, ***P < 0.001, vs. OE Ctrl.