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. 2020 Dec 12;11(12):1060. doi: 10.1038/s41419-020-03282-3

Table 1.

Primers for real-time PCR.

Genes or binding sites Primers (5′–3′)
FZD5-forward TCCTCTGCATGGATTACAACC
FZD5-reverse GACACTTGCACACGAACG
EXO1-forward GCTCGGCTAGGAATGTGCAGAC
EXO1-reverse CCCACGCAGTGATGACAGGTAG
PLK4-forward CCTTCTCAGAAAATGAAGCTCG
PLK4-reverse TCATGTGGCATTTTCAGTTGAG
RFC4-forward AAACCACCCGATTCTGTCTTAT
RFC4-reverse CTTGGCAATGTCTAGTAATCGC
CD133-forward GTGGCGTGTGCGGCTATGAC
CD133-reverse CCAACTCCAACCATGAGGAAGACG
EPCAM-forward GTCTGTGAAAACTACAAGCTGG
EPCAM-reverse CAGTATTTTGTGCACCAACTGA
ALDH1A2-forward TGCTGATGCTGACTTGGACTATGC
ALDH1A2-reverse CCGCTCCACGCTTCTTCTCAC
POU5F1-forward GATGTGGTCCGAGTGTGGTTCTG
POU5F1-reverse CGAGGAGTACAGTGCAGTGAAGTG
GAPDH-forward CAGGAGGCATTGCTGATGAT
GAPDH-reverse GAAGGCTGGGGCTCATTT
BRCA1 BS1-forward CTTTTACGTCATCCGGGGGC
BRCA1 BS1-reverse CGCGCAGTCGCAGTTTTAAT
BRCA1 BS2-forward GCAGTGGTGCAATCTGGG
BRCA1 BS2-reverse GGTGGATCACGAGGTCAAG
BIRC5 BS1+BS2-forward TGGTAATGCCTTCAACTT
BIRC5 BS1+BS2-reverse TCTCCCCAACCTACTTTC