Table 3. Quantification of cell surface expression of CA125 and EpCAM, and intracellular expression of CK7 in ascites cell samples by determination of the ratio of the median fluorescence intensity (MFI) after staining with anti-CA125, anti-EpCAM or anti-CK7 antibody relative to staining with isotype control antibody.

Patient sample	CA125		EpCAM		CK7
	MFI ratio	% positive	MFI ratio	% positive	MFI ratio	% positive
1	1.35	47	0.93	3.4	10.9	80
2	1.26	42	0.96	1.6	9.65	89
4	1.30	47	0.98	3.3	9.59	85
12	3.01	90	1.41	23	15.5	93
14	2.48	87	1.28	22	6.69	87
SK-OV-3	1.85	47	339	99	1.50	36

Moreover, the percentage of cells stained positive for the different markers was determined. Abbreviations: MFI, median fluorescence intensity.