Figure 4.

Reduced ICA‐069673 sensitivity of Kv7.2/Kv7.3[T313I] heteromeric channels. A, Example currents of two‐electrode voltage‐clamp recordings. Oocytes were depolarized to +20 mV and repolarized for 12 s in a step‐down manner (−20 mV per sweep), followed by another +20 mV depolarizing pulse to determine instantaneous current at −100 mV. Currents within the dashed box are illustrated on an expanded time scale in the right panels, showing the assessment of instantaneous current levels in different experimental conditions. B, Fractional instantaneous current after incubation with 100 μmol/L ICA‐069673 was measured as indicated by the arrows in panel (A), data are shown as mean ± SEM. C, Fractional instantaneous current (repolarization voltage of −100 mV) for various combinations of Kv7.2, Kv7.3, and Kv7.3[T313I], shown on a cell‐by‐cell basis (Kruskal‐Wallis one‐way ANOVA on ranks, * indicates P < 0.05 relative to Kv7.2 homomeric channels)