Figure 5.

Variable outcomes of homologous selectivity filter mutations in Kv7.2 and Kv7.3. A, Current magnitude was measured at +20 mV in Xenopus oocytes injected with Kv7.2 + Kv7.3, or various combinations of channel mutants and their wild‐type counterpart, as indicated. Currents were recording 48‐56 h after injection (n = 10 per condition, one‐way ANOVA on ranks and Tukey post hoc test, # indicates P < 0.05 relative to Kv7.2/Kv7.3, * indicates P < 0.05 relative to Kv7.2/Kv7.3[T313I]). B, Instantaneous tail current magnitudes were recorded as described in Figure 4 in the presence of 100 μmol/L ICA‐069673, for all possible combinations of injected channels (note that Kv7.3 + Kv7.2[T274I] did not yield current sizes that could be confidently assessed or analyzed)