Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Dec 27;3(1):9–19. doi: 10.31662/jmaj.2019-0027

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © Japan Medical Association

JMA Journal is an Open Access journal distributed under the Creative Commons Attribution 4.0 International License. To view the details of this license, please visit (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Figure 1. — Intestinal organoids are generated from either isolated intestinal stem cells or human embryonic stem cells (ESCs)/induced pluripotent stem cells (iPSCs) in vitro. Lgr5+ cells isolated from collected tissue are embedded in a three-dimensional (3D)-Matrigel suspension culture and grow into organized epithelial organoids. Pluripotent stem cells (PSCs) differentiate into CDX2 expressing spheroids containing endoderm and mesoderm and subsequently grow in Matrigel (HIOs). Mini-Guts are self-organized from PSCs in a xenogeneic-free culture condition. The Mini-Guts contain intestinal epithelium and mesenchymal layers, with the epithelial layer as an outer layer. LGR5+, leucine-rich repeat-containing G-protein-coupled receptor 5 positive; LS, luminal side.