Fig. 3.

Chemical structures and IC₅₀ (μM) values for selected hits in the linked Zika virus protease (Protease) and Zika virus infection assay in NSCs. (A) FLAP inhibitors, (B) γ-secretase modulators, (C) tetracyclines, (D) AMPK activator, (E) farnesoid X receptor agonists, (F) liver X receptor agonist, (G) retinoic acid analog, (H) mineralocorticoid receptor antagonist, (I) compound from QSAR screen. An asterisk (*) indicates IC₅₀ values are a single data point from qHTS. All other values are an average of at least three independent experiments from the confirmation testing of commercial compounds. Protease and NSC IC₅₀ values were calculated in GraphPad Prism using the equation: Log(inhibitor) vs. normalized response–variable slope.