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. 2020 Nov 23;117(49):31510–31518. doi: 10.1073/pnas.2018415117

Fig. 2.

Fig. 2.

INR confers inceptin signaling responses in heterologous model N. benthamiana, corresponding with cowpea response variation. (A–C) Ligand-dependent ROS production following the heterologous expression of receptors in N. benthamiana. Shone are relative luminescence units (RLU), minutes (min) after treatment with H2O or 1 μM peptides elf-18 or Vu-In. (A) Peptides applied to plants expressing elf-18 receptor (elongation factor-Tu; EFR). (B) Peptides applied to plants expressing INR (INR-Vu; Vigun07g219600). (C) Cumulative RLU counts from A and B after indicated peptide treatments. Bars show average of n = 8 leaf discs ± SEM. (D) Receptor-dependent ethylene production in N. benthamiana after treatment with H2O or 1 μM peptides. Bars show average of n = 6 leaf discs ± SEM. (E) Vu-In-A/H2O induced ethylene response ratio in select cowpea germplasm (Top), with corresponding ROS responses when INR alleles are expressed in N. benthamiana (Bottom). Gray boxes indicate accessions with GG allele at mapped marker 2_22561, and white boxes indicate AA allele. See SI Appendix, Table S2 for a full list of UCR Minicore responses. Bars show average of n = 8 leaf discs ± SEM. Within C–E, different letters indicate significant differences (all ANOVAs P < 0.05; Tukey HSD, α = 0.05).