Fig. 2.

Diurnal variation and effect of activity in muscle protein synthesis. Larvae raised under LD that were either untreated (Control, blue) or anesthetized (Tricaine, red) at 3 dpf, and then treated with OPP for 2 h from ZT0 to ZT2 (Day) or ZT12 to ZT14 (Night). (A) Nascent proteins visualized by confocal microscopy. Box schematizes amino acid (AA) chain termination by OPP and detection with PA. Larvae treated with CHX and OPP from 3 dpf were analyzed at ZT2 as a negative control. (B and C) Quantification of OPP−PA fluorescence of somite 17 (n = 9 fish from three biological replicates). Signals were normalized to Control ZT0 to ZT2 samples (B), and compared after subtraction of CHX background (C). Symbols distinguish fish from different lays.