Fig. 4.

GFP^hi cells resolve into four subgroups of quiescent neural stem cells. (A) NSC-specific transcription factors are enriched in the GFP^hi subgroups (all SEs < 0.6; units are normalized values scaled to 10,000 counts/cell). (B) Neural progenitor-specific transcription factors are enriched in GFP^lo subgroups (all SEs < 0.2; units are normalized values scaled to 10,000 counts/cell). (C) GFP^hi and GFP^lo subgroup-specific gene signatures improve resolution of published SVZ cell-sequencing profiles. Transcriptomes of 155 cells published in Llorens-Bobadilla et al. (23) were analyzed with our signatures. Each column represents one cell. (D, D¹, E, and E¹) FACS analysis showed (D and D¹) approximately one-half of GFP^hi;CD95+ cells are also CD133+, and (E and E¹) about 20% of the GFP^hi;CD133+ cells are CD95+. (F) Pseudotime analysis provides a nearest neighbor random walk of single-cell transcriptional profiles revealing a linear progression from GFP^hi:H1 through GFP^lo:L2. (G) H0 cells preferentially reside on the ventral aspect of adult SVZ as defined by Allen Brain Atlas expression profiles. * indicates P values <0.05. See also SI Appendix, Fig. S4.