Fig. 5.

H0 cells have unique stem-like properties. (A) Expression of four H0-specific genes (Tmem212, Dynlrb2, Fam183b, and 1110017D15rik) was verified by qRT-PCR in single-cell sequencing samples. Gene expression levels were normalized to UNB cells. Mean ± SEM, n = 2. (B) CD95-Fas receptor antibody labels one subgroup of GFP^hi and one subgroup of GFP– cells. All of the numbers represent the percentage of each population among the whole SVZ. (C) GFP^hi;CD95+ cells have enriched expression of the H0-specific genes. qRT-PCR was performed with GFP and CD95 sorted cells for the H0-specific genes. Gene expression levels were normalized to GFP^hi cells. Mean ± SEM, n = 2. (D and D¹) Representative images and quantification of doublet formation by GFP and CD95 sorted cells at 24 h. Note that GFP^hi;CD95– cells are more efficient at forming doublets (Scale bar, 10 μm.) (E and E¹) Representative images and quantification of sphere formation of GFP and CD95 sorted cells. Note that, reminiscent of Fig. 2 C and D, GFP^hi;CD95+ cells, which were inefficient in doublet formation at 24 h, are most efficient at forming neurospheres 6 d later. Note also that GFP–;CD95+ cells do not form neurospheres. (Scale bar, 100 μm.) Mean ± SEM, n = 3 biological replicate mice for each group in D and E¹. **** in D¹ and E¹ indicates P values <0.0001. See also SI Appendix, Fig. S5.