Fig. 3. Clonal relationships of intestinal plasma B cell clusters in health and ulcerative colitis.

(A) Quantitation of IgA1⁺, IgA2⁺, and IgG1⁺ cells within each plasma cell cluster, determined using scBCR-seq data. (B) Absolute number and percentage of cells from UC patients or healthy individuals are shown for each immunoglobulin isotype (IgA1⁺, IgA2⁺, IgD⁺, IgG1⁺, IgG2⁺, and IgM⁺ only; very few IgG3⁺, IgG4⁺, or IgE⁺ cells were detected). (C) t-SNE plots of plasma cell clusters, colored by cluster identity, with red lines indicating BCR clonotypes shared among clusters and line weight representing number of shared clonotypes, for healthy individuals (left) vs. UC patients (right).