FIG 2.
Cu-dependent defects in growth and viability. GAS strains were cultured with added Cu as indicated. (A) Growth. Cultures (n = 3) were grown in microtiter plates and OD600 values were recorded every 20 min. Cu treatment suppressed growth of ΔcopA cultures (P = 0.034 for 5 μM Cu, P < 0.0001 for 10 μM Cu). (B) Plating efficiency. Cultures (n = 3) were plated out at the indicated time points and the number of CFU was enumerated. Cu treatment suppressed plating efficiency of the ΔcopA cultures (P < 0.0001 for both 0.5 and 5 μM Cu). (C) Total ATP levels. Cultures (n = 5) were sampled at the indicated time points and total ATP levels were determined. Cu treatment suppressed ATP production in the ΔcopA cultures (P < 0.0001). All statistical analyses were versus 0 μM Cu.