FIG 10.

Domain structure of W909_08750 and swimming motility of EC1 and its derivatives. (A) Predicted domain architectures of W909_08750. Domains of the proteins were predicted and represented using DOG 2.0 software (67). YcgR is involved in motility control in enterobacteria. PilZ is a receptor domain specific for c-di-GMP (68). (B and C) Swimming motility of EC1, 7ΔPDE, and 7ΔPDEΔW909_08750. Cultures grown overnight were adjusted to the same cell density (OD₆₀₀ = 2.0), and 1 μl was inoculated onto swimming agar plates. Data were collected after 18 h at 28°C. Dotted lines indicate the wild-type level of swimming motility. Experiments were repeated three times in triplicates. Each sample was statistically compared to every other sample; the same lowercase letters indicate samples that are not significantly different, and different letters indicate significant differences (P < 0.0001), as determined by one-way ANOVA with a multiple-comparison test.