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. 2020 Dec 8;11(6):e03012-20. doi: 10.1128/mBio.03012-20

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Copyright © 2020 Park et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 5 — Rescue of FtsL^Δ^1-30 by ftsI expression. (A) ftsI expression rescues FtsL^Δ^1-30. To test if overexpression of ftsI could rescue FtsL^Δ^1-30, PK4-1 (ftsL::kan/pKTP108 [repA^TS::ftsL]) was transformed with a plasmid expressing ftsL^Δ^1-30 (pKTP107X/P_ara::ftsL^Δ^1-30-6Xhis) and a plasmid expressing ftsI (pSEB420/P₂₀₄::ftsI) or ftsW (pSEB429/P₂₀₄::ftsW) under an IPTG-inducible promoter. The strains were spot tested on plates at 37°C to deplete ftsL. In addition, arabinose was added to induce ftsL^Δ^1-30, and increasing concentrations of IPTG were added to induce ftsI or ftsW. (B) ftsI overexpression cannot rescue a dominant negative ftsL allele. SD399 (ftsL::kan/pSD256 [repA^ts::ftsL]) was transformed with pSD296-2 (P_ara::ftsL^L86F/E87K) and pSEB420 (P₂₀₄::ftsI) or pSEB429 (P₂₀₄::ftsW). Transformants were spot tested at 37°C (to deplete ftsL) on plates containing 0.2% arabinose (to induce ftsL^L86F/E87K) and increasing concentrations of IPTG (to induce ftsI or ftsW).