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. 2020 Dec 8;11(6):e03012-20. doi: 10.1128/mBio.03012-20

FIG 7.

FIG 7

Interaction between FtsL and FtsWI assessed with the BACTH system. (A) Effect of cytoFtsL on the interaction between FtsL and FtsWI. Strain DHM1 was transformed with plasmids carrying various alleles of ftsL (pUT18C derivatives) and plasmids expressing ftsW or ftsI (pKT25 derivatives). Three transformants were picked and spot tested for each pair of constructs. The positive control contained plasmids pUT18C-zip and pKT25-zip, whereas the negative control contained the corresponding empty vectors. (B) The effect of activation and dominant negative mutations in ftsL on the interaction of FtsLΔ1-30 or FtsLL24K/I28K with FtsW and FtsI. Three transformants from each transformation of DHM1 with plasmids carrying the ftsL and ftsW or ftsI alleles were spotted on plates containing the color indicator. The ftsL alleles were contained in pUT18C, and the ftsW and ftsI alleles were in pKT25. The plates were photographed after overnight incubation.