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. 2020 Dec 8;11(6):e03012-20. doi: 10.1128/mBio.03012-20

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Copyright © 2020 Park et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 8 — Activation mutations allow a malF-ftsL fusion to complement ΔftsL in the presence of a ftsW-^cytoftsK fusion. Plasmid pKTP103 (P_tac::malF^1-37-ftsL^58-121-6xhis) was introduced into an ftsL depletion strain (SD439 ftsL::kan/pSD296 [P_ara::ftsL]) in the presence of a plasmid constitutively expressing a FtsW-FtsK^cyto fusion without or with an activation mutation (pND16 [P_ftsK::ftsW-^cytoftsK] or pND16* [P_ftsK::ftsW^M269I-^cytoftsK], respectively). The strains were spot tested on plates without arabinose (to deplete WT ftsL) and in the presence of IPTG (to induce malF-ftsL) (with or without the activation mutations [ftsL^E88K and ftsL^G92D]). The cartoon to the right depicts the activity of the FtsL constructs.