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. 2020 Dec 1;11(6):e03254-19. doi: 10.1128/mBio.03254-19

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Copyright © 2020 Sun et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 3 — Interaction of MmsA and STING depends on the N-terminal region of both proteins. (A) Schematic diagram of STING and its truncation mutants. pMyc-human-STING or its mutants pSTING^N-Region and pSTING^C-Region were individually transfected into HEK293T cells along with pFlag-MmsA. Cell lysates were immunoprecipitated with the anti-Flag antibody and then immunoblotted with the indicated antibodies. (B) Schematic diagram of MmsA and its truncation mutants. pFlag-MmsA or its mutants, pFlag-MmsA^1-251aa and pFlag-MmsA^1-451aa, were individually transfected into HEK293T cells along with pMyc-human-STING. Cell lysates were immunoprecipitated with the anti-Myc antibody and then immunoblotted with the indicated antibodies.