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. 2020 Dec 1;11(6):e03254-19. doi: 10.1128/mBio.03254-19

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Copyright © 2020 Sun et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 6 — MmsA interacts with p62 for STING autophagy degradation. (A) HEK293T cells were transfected with Flag-STING and Myc-p62, with or without Flag-MmsA, for 48 h. Cell lysates were immunoprecipitated with the anti-Myc antibody, followed by immunoblotting with the indicated antibodies. (B) Cell lysates of RAW-MmsA and RAW-Vector were immunoprecipitated with the anti-p62 antibody and analyzed by immunoblotting with the indicated antibodies. (C) RAW-MmsA and RAW-Vector cells were transfected with siRNA-control and siRNA-p62 for 48 h and analyzed by immunoblotting with the indicated antibodies. (D) HEK293T cells were transfected with pFlag-MmsA or its mutants along with pMyc-p62 for 48 h. Cell lysates were immunoprecipitated with the anti-Flag antibody and then immunoblotted with the indicated antibodies. (E) HEK293T cells were transfected with pMyc-p62, pFlag-MmsA, and pMyc-human-STING or Myc-Vector for 48 h. Cell lysates were immunoprecipitated with the anti-Flag antibody and then immunoblotted with the indicated antibodies.