Figure 2.

Partial correlation-based network inference suggests that the ERK/AKT signaling network is significantly rewired during EMT. (A) Overview of an EMT model (top) including an illustration of the visual phenotype and a molecular definition of epithelial and mesenchymal cells measured by mass cytometry at various time points (bottom). (B) Partial correlation (ρ_XY·Z) analysis of phosphoproteins is used to infer the different network structures in epithelial and mesenchymal cells sampled across replicates. The threshold for accepting an edge between X and Y is defined as ρ_XY·Z ≥ |0.1|. All values are Fisher z-transformed. (C) Time dependence of signaling strength in canonical signaling edges. Blue and green represent epithelial and mesenchymal phenotypes, respectively. Error bars represent standard deviation of partial correlation across replicates.