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. 2020 Nov 30;11:617237. doi: 10.3389/fphys.2020.617237

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Copyright © 2020 Zhu, Zheng, Xu, Wu, Song and Ge.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Silencing of TOR pathway-related gene changes Vg accumulation in ovarioles of adult females at 7 DAE. Left panels (A,D,G,J) show DAPI-stained nuclei; middle panels (B,E,H,K) show Vg protein detected with goat anti-rabbit IgG-labeled with Dylight 488 (green); right panels (C,F,I,L) showed merged images. Fluorescent images were captured with a Zeiss LSM 780 confocal microscope (Carl Zeiss MicroImaging, Göttingen, Germany). Fc, follicular cell; Oo, oocyte. Bars, 100 μm.