Figure 4.

Lactobacillus spp. are susceptible to synthetic miR-21. (a) Bacterial colonies of L. reuteri DSM 17938 and L. reuteri ATCC PTA 6475 exposed to either sterile water (control), synthetic miR-21 (h-miR-21) or synthetic scrambled miR-21 (h-miR-21-scr). (b) Colony-forming unit (CFU)/ml of L. reuteri DSM 17938 and L. reuteri ATCC PTA 6475 exposed to either sterile water (control), synthetic miR-21 (h-miR-21) or synthetic scrambled miR-21 (h-miR-21-scr). Representative numbers of bacterial colonies cultured on MRS agar. Mean values were calculated as fold change versus controls with error bars ± SEM of 6–10 individual experiments. Statistical analysis performed with ANOVA Tukey’s multiple comparisons test. **p < .01, ****p < .0001