Figure 1.

Effect of LAA in H9c2 cardiomyoblasts. A, Cell proliferation in LAA‐treated H9c2 cells. B, Flux analysis of LAA‐treated H9c2 cells. C, Effect of LAA on mitochondrial respiration, ATP production, and proton leak. D, Effect of LAA on mitochondrial membrane voltage (evaluated using TMRE), mitochondrial volume (evaluated using Mitogreen) and oxidative stress (evaluated using DHR). E, Effect of LAA on glycolytic activity (determined by ECAR measurement). F, Effect of LAA on cell energy phenotype profile. Error bars, standard error from 3 trials. The statistical significance was calculated by Student t test. C, control; DHR, dihydrorhodamine 123; ECAR, extracellular acidification rate; LAA, lauric acid; OCR, oxygen consumption rate; TMRE, tetramethyl rhodamine