Table 1.
Components of medium for treatment
| Component | Medium a | ||
|---|---|---|---|
| D‐MEM a | Ascites added b | CM added c | |
| Glucose (mg/dL) | 450 ± 2 | 361 ± 8 | 378 ± 6 |
| Pyruvate (mg/dL) | 11 ± 0.1 | 9 ± 1 | 9 ± 1 |
| Glutamine (mg/dL) | 58 ± 0.2 | 49 ± 4 | 50 ± 3 |
| Lactate (pmol) | 0 | 6.1 ± 1.2 | 1.2 ± 0.2 |
| HMGB1 (μg/mL) | ND d | 12 ± 0.8 | ND |
| TNFα (pg/mL) | ND | 9 ± 0.1 | ND |
a
Regular medium: D‐MEM (WAKO, Osaka, Japan) supplemented with 10% fetal bovine serum.
b
Regular medium with added 30% (v/v) mouse ascites of the cachexia model.
c
Regular medium with added 30% (v/v) culture medium obtained from culturing H9c2 for 48 h.
d
ND, not detected.