FIGURE 3.

Combined therapies of THC and anti‐PD‐1/PD‐L1 Ab synergistically augment the gp70‐specific T cell induction and the anti‐tumor effect. The combined therapy of THC and anti‐PD‐1 Ab (A‐C) or anti‐PD‐L1 Ab (D) given ip on days 7, 10, and 13, was performed. On day 16 after implantation of DsRed‐labeled MC38 to C57BL/6 mice, tumor was collected from the mice. The expression of PD‐L1 on MC38 cells and the expression of PD‐1 on tumor‐infiltrating CD8⁺ T cells were evaluated by flow cytometry (A). On day 22, the combined therapy of THC and anti‐PD‐1 Ab synergistically inhibited tumor growth compared with each single treatment (B) and the combined therapy augmented the induction of gp70‐specific T cells in tumor tissues (C: left) and dLNs (C: right) by the same experiment as Figure 1B. The combined therapy of THC and anti‐PD‐L1 Ab synergistically inhibited tumor growth compared with each single treatment (D). Mean tumor size ± SD. Five mice were included in each group (4 mice were included in the control group). All results in this figure are from 2 independent experiments. Error bars indicate SD. *P < .05, **P < .01 using a t test