Figure 3.

Physiological characteristics of NTCD-035 C. difficile is similar to those of CD196 and R20291 except for the capacity to produce toxin

(a) Growth curves of C. difficile strains as assessed by optical density at 600 nm. (b) Toxin production as assessed by Vero cell assay. C. difficile was cultured anaerobically in BHIS broth at 37°C. Aliquots were taken from liquid culture at various time points and assayed for toxin activity. (c) Sporulation rates of C. difficile strains. C. difficile was cultured anaerobically in BHIS broth at 37°C. Aliquots were taken at various time points, heat killed at 65°C for 60 minutes and anaerobically plated on BHISTA plates to quantify the number of spores. (d-e) Growth curves of C. difficile strains as assessed by optical density at 600 nm (d) in the presence of deoxycholic acid, (e) or lithocholic acid. (f) Antibiotic sensitivity assay assessing C. difficile sensitivity to metronidazole, neomycin, vancomycin, and clindamycin