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. 2020 Dec 14;11(12):1066. doi: 10.1038/s41419-020-03271-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a, b The expression of ATF3 and α-SMA was assessed by qRT-PCR analysis and immunoblot in livers from mice treated with CCl₄ for 8 weeks. c The expression of ATF3 was examined in CCl₄-induced fibrotic livers using IHC staining, 10× and 40× magnification. d, e The expression of ATF3 and α-SMA was assessed by qRT-PCR analysis and immunoblot in livers from mice that underwent BDL for 21 days. f The expression of ATF3 was examined in BDL-induced fibrotic livers using IHC staining, 10× and 40× magnification. g, h The expression of ATF3 was assessed by qRT-PCR analysis and immunoblot in liver samples of healthy people and fibrotic patients. The data were expressed as the mean ± SD for at least triplicate experiments, GAPDH was used as an internal control; *p < 0.05.