Fig. 6. lnc-SCARNA10 forms a positive feedback loop with ATF3.
a LX-2 cells were infected with ATF3-shRNA1/2 for 72 h. The expression of lnc-SCARNA10 was detected by qRT-PCR. b The expression of lnc-SCARNA10 was detected in LX-2 cells infected with lenti-ATF3 or lenti-control by qRT-PCR. c LX-2 cells were treated with TGF-β and ChIP analyses were performed on SCARNA10 promoter regions, using anti-ATF3 antibody. Enrichment was shown relative to input. d–f LX-2 cells were infected with lnc-SCARNA10 shRNA1/2 or lenti-lnc-SCARNA10 for 72 h. The expression of ATF3 was detected by qRT-PCR and western blot. GAPDH was used as an internal control. The data were expressed as the mean ± SEM for at least triplicate experiments. GAPDH was used as an internal control; *p < 0.05.