Table 3.
qRT-PCR primers for analysis of transcript levels.
| Gene symbol | Forward 5′–3′ | Reverse 5′–3′ |
|---|---|---|
| Atf3 (mouse) | CTCTGCCATCGGATGTCCTC | GTTTCGACACTTGGCAGCAG |
| Gapdh (mouse) | GGCATGGACTGTGGTCATGAG | TGCACCACCAACTGCTTAGC |
| Col1α1 (mouse) | ATCGGTCATGCTCTCTCCAAACCA | ACTGCAACATGGAGACAGGTCAGA |
| Col3α1 (mouse) | TGCTCCAGTTAGCCCTGCAA | GGTCCTGCAGGCAACAGTGGTTC |
| Col4α5 (mouse) | CTCCCTTACCGCCCTTTTCTC | AGGCGAAATGGGTATGATGGG |
| α-SMA (mouse) | TCGGATACTTCAGCGTCAGGA | GTCCCAGACATCAGGGAGTAA |
| TIMP1 (mouse) | TCCGTCCACAAACAGTGAGTGTCA | GGTGTGCACAGTGTTTCCCTGTTT |
| MMP2 (mouse) | GTGTTCTTCGCAGGGAATGAG | GATGCTTCCAAACTTCACGCT |
| Pcna (mouse) | TTTGAGGCACGCCTGATCC | GGAGACGTGAGACGAGTCCAT |
| Bax (mouse) | TTGCTGATGGCAACTTCAAC | GATCAGCTCGGGCACTTTAG |
| Mcp1 (mouse) | GTTAACGCCCCACTCACCTG | GGGCCGGGGTATGTAACTCA |
| Bcl2 (mouse) | GCTGGGATGCCTTTGTGGAACT | CAGAGACAGCCAGGAGAAATCAAAC |
| ATF3 (human) | CTAACCTGACGCCCTTTGTC | ACTCTTTCTGCAGGCACTCC |
| GAPDH (human) | ACCCAGAAGACTGTGGATGG | TTCAGCTCAGGGATGACCTT |
| COL1α1 (human) | AACCAAGGCTGCAACCTGGA | GGCTGAGTAGGGTACACGCAGG |
| COL3α1 (human) | AGCCTTGCGTGTTCGATAT | GAAGATGTCCTTGATGTGC |
| COL4α5 (human) | TTCAGCGTTTCTGACTGAGG | AGAGCATCCAGCCATTCATT |
| α-SMA (human) | GCCATGTTCTATCGGGTACTTC | CAGGGCTGTTTTCCCATCCAT |
| TIMP1 (human) | GGGGCTTCACCAAGACCTAC | GGAAGCCCTTTTCAGAGCCT |
| MMP2 (human) | GTGTTCTTCGCAGGGAATGAG | GATGCTTCCAAACTTCACGCT |
| MMP9 (human) | CCTTGTGCTCTTCCCTGGAG | GGCCCCAGAGATTTCGACTC |
| SMAD3 (human) | CCCCAGCACATAATAACTTGG | AGGAGATGGAGCACCAGAAG |
| SCARNA10 (human) | CCAGGGAGGAATTGTGGTAA | TCTGTGTGTCATCTCTCAGTGG |