Table 2.
miRNA ID | VEX |
TEX |
||
---|---|---|---|---|
8% PEG | TEI | 8% PEG | TEI | |
gga-miR-146b-5p | 21.84 ± 0.51 | 20.74 ± 0.52 | 20.36 ± 0.89 | 20.8 ± 1.07 |
MDV1-miR-M6-5p | 26.17 ± 0.58 | 25.31 ± 0.01 | 24.82 ± 0.71 | 25.53 ± 0.94 |
gga-miR-99a-5p | 26.24 ± 1.19 | 25.23 ± 0.11 | 26.13 ± 0.04 | 25.38 ± 0.64 |
gga-miR-21-5pa | 26.85 ± 1.04 | 24.18 ± 0.46 | 22.26 ± 0.01 | 24.40 ± 1.47 |
MDV1-miR-M4-5p | 27.40 ± 0.16 | 25.13 ± 0.36 | 25.21 ± 0.08 | 25.53 ± 0.96 |
MDV1-miR-M8-5pa | 28.83 ± 1.1 | 26.04 ± 0.38 | 24.13 ± 0.12 | 26.04 ± 0.57 |
MDV1-miR-M12-3pa | 29.37 ± 0.73 | 26.43 ± 0.59 | 25.40 ± 0.33 | 26.01 ± 0.81 |
gga-miR-2188-5p | 30.41 ± 0.50 | 28.20 ± 0.54 | 28.90 ± 0.22 | 28.89 ± 1.23 |
gga-miR-27b-3pa | 30.47 ± 0.70 | 28.15 ± 0.31 | 29.38 ± 0.09 | 28.20 ± 0.46 |
gga-miR-10b-5pa |
33.54 ± 0.85 |
28.83 ± 0.58 |
29.34 ± 0.44 |
29.95 ± 0.44 |
Average Ct | 28.11 ± 3.02 | 25.9 ± 2.24 | 25.32 ± 2.85 | 26.08 ± 2.43 |
miRNA abundance differences in VEX were significant different between the purification methods.