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. 2020 Dec 11;6(12):e05669. doi: 10.1016/j.heliyon.2020.e05669

Table 2.

Exosomal miRNA Ct values (±SD) as Detected by qRT-PCR.

miRNA ID VEX
TEX
8% PEG TEI 8% PEG TEI
gga-miR-146b-5p 21.84 ± 0.51 20.74 ± 0.52 20.36 ± 0.89 20.8 ± 1.07
MDV1-miR-M6-5p 26.17 ± 0.58 25.31 ± 0.01 24.82 ± 0.71 25.53 ± 0.94
gga-miR-99a-5p 26.24 ± 1.19 25.23 ± 0.11 26.13 ± 0.04 25.38 ± 0.64
gga-miR-21-5pa 26.85 ± 1.04 24.18 ± 0.46 22.26 ± 0.01 24.40 ± 1.47
MDV1-miR-M4-5p 27.40 ± 0.16 25.13 ± 0.36 25.21 ± 0.08 25.53 ± 0.96
MDV1-miR-M8-5pa 28.83 ± 1.1 26.04 ± 0.38 24.13 ± 0.12 26.04 ± 0.57
MDV1-miR-M12-3pa 29.37 ± 0.73 26.43 ± 0.59 25.40 ± 0.33 26.01 ± 0.81
gga-miR-2188-5p 30.41 ± 0.50 28.20 ± 0.54 28.90 ± 0.22 28.89 ± 1.23
gga-miR-27b-3pa 30.47 ± 0.70 28.15 ± 0.31 29.38 ± 0.09 28.20 ± 0.46
gga-miR-10b-5pa
33.54 ± 0.85
28.83 ± 0.58
29.34 ± 0.44
29.95 ± 0.44
Average Ct 28.11 ± 3.02 25.9 ± 2.24 25.32 ± 2.85 26.08 ± 2.43
a

miRNA abundance differences in VEX were significant different between the purification methods.