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Determination of peptide binding affinity to HLA-A2. (A) The mean fluorescence index (MFI) values of HLA-A2 molecules on T2 cells after incubating with 57 pairs HLA-A*0201 restricted wildtype (WT) and mutant (MT) peptides individually. (B) The MFI values of HLA-A2 molecules from 18 pairs HLA-A*0201 WT and MT peptides in a repetitive experiment. (C) The MFI values of HLA-A2 molecules at different concentrations of 12 MT peptides. (D) The Kd values of 12 MT peptides.
