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. 2020 Nov 30;11:593103. doi: 10.3389/fimmu.2020.593103

Figure 5.

Figure 5

Inhibition of fatty acid synthesis suppressed IFN-γ production and increased Foxp3 expression in T-bet+Foxp3+ cells in memory CD4+ T cells in SLE. CD45RA- memory CD4+ T cells from peripheral blood of HDs and patients with SLE were stimulated for 72 h with anti-CD3 Abs and anti-CD28 Abs or with 2DG (3 mM), rapamycin (10 nM), C75 (10 μM) or etmoxir (10 or 50 μM). (A) Expression of Foxp3 and IFN-γ in CD45RA- memory CD4+ T cells from patients with SLE. Representative experiment depicting Foxp3 and IFN-γ expression in SLE samples (upper panel) and histogram of T-bet expression (lower panel) in three independent experiments. (B) Bar graph of the percentage of Foxp3+IFN-γ+ cells among CD45RA-CD4+ cells from HDs and patients with SLE. Data are mean ± SD of three experiments using CD45RA- memory CD4+cells from each six different donors of HDs and SLE patients (paired T test). (C) Representative data of Foxp3 expression gated in T-bet+Foxp3+ cells in CD4+CD45RA- cells from SLE samples in three independent experiments. (D) Concentration and fold change of cytokines by cytometric bead array in HDs and SLE samples. Data are mean ± SD of using CD45RA- memory CD4+cells from each different donors of HDs and SLE patients (paired T test). *p < 0.05. **p < 0.01. ***p < 0.001. ****p < 0.0001. n.s., not significant.