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. 2020 Nov 30;10:573097. doi: 10.3389/fcimb.2020.573097

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Copyright © 2020 Assunção, Moraes, Soares, Silva-Bailão, de Siqueira, Baeza, Báo, Soares and Bailão

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Influence of Zn availability in H. capsulatum survival in macrophages. (A) Relative expression of Zap1 and Zrt1 in H. capsulatum during infection overtime. (B) Fungal burden of macrophages treated with (Hc + MØ), ZnSO4 (Hc + MØ + ZnSO4), and DTPA (Hc + MØ + DTPA) conditions. Control: H. capsulatum incubated in macrophage medium; Infection: macrophages infected with H. capsulatum yeasts. (C) Viability of macrophages in RPMI medium was measured by trypan blue dye. Statistical analysis was performed using t-test, one-way ANOVA, and the Tukey multiple comparison test with *p ≤ 0.05. All experiments were carried out in triplicates.