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. 2020 Nov 30;11:588500. doi: 10.3389/fimmu.2020.588500

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Copyright © 2020 Wu, Zhu, Tian, Tang, Guo, Ma, Xu and Wang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Extraction and identification of G-exo. (A) G-MDSCs were sorted from mouse spleens using immunomagnetic beads. The expression of Ly-6G and CD11b was analyzed by flow cytometry. (B) The expression of CD63, CD9 and calnexin was assessed by Western blot analysis. (C) Representative transmission electron micrograph of G-exo (scale bar = 200 nm). (D) Particle size distribution of G-exo analyzed by nanoparticle tracking analysis (NTA). The presented data are from one of three independent experiments.