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Lysine was the most consumed essential amino acid (EAA) in vivo in ER+ breast cancer patients. Microdialysis was used for sampling of extracellular essential AAs (EAAs) from the live tissue of ER+ BC patients before surgery. One catheter was inserted within the breast cancer and another in normal adjacent breast tissue. EAAs were quantified by UHPLC-ESI-MS/MS as reported in materials and methods. Wilcoxon signed-rank test, *P < 0.05 and ***P < 0.001.
