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. 2020 Nov 30;11:593492. doi: 10.3389/fphar.2020.593492

FIGURE 2.

FIGURE 2

LMW agonists do not alter the endosomal organization of FSHR (A) Representative TIR-FM images of FLAG-FSHR expressing HEK 293 cells treated with either DMSO, FSH (10 nM), B3 or T1 (10 μM) for 5 min. Scale bar = 10 μm. (B) Quantification of FSHR endosomes in stimulated cells from (A); n = 24 cells/condition collected across three independent experiments. One-way ANOVA: **p < 0.01. (C) TIR-FM images of FLAG-FSHR (cyan) and endogenous APPL1 (magenta) in cells stimulated with either FSH (10 nM), B3 or T1 (10 μM) for 5 min. Scale bar = 1 μm. (D) Quantification of FSHR endosomes positive for APPL1 from (C); n = 10–12 cells/condition collected across three independent experiments. (E) TIR-FM images of FSHR (cyan) and endogenous EEA1 (magenta) in cells stimulated with either FSH (10 nM), B3 or T1 (10 μM) for 5 min. Scale bar = 1 μm. (F) Quantification of FSHR endosomes positive for EEA1 from (E); n = 11–12 cells/condition collected across three independent experiments.